FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16489789
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC575180.3488097998100521No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT257120.1559267980930502No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA252470.15310687116736302No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT187600.1137673744642821No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT178800.10843073856190641No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA175350.10633853471381592No Hit
TGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT166390.10090486906776067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC95000.0114.970091
CGGGCGC284450.0114.2298361
CGGCTAA5100.0110.873021
CGGTTAA734600.0109.894821
CGGGTAC111150.0107.047311
GGGCGCG334150.0103.335162
CGGATGC10250.096.395471
GGGAGGC477550.096.0457842
CGGTCTA750.095.2340851
GGCGCGG351800.095.105773
CGGGTAT336600.094.869811
CGGGTGT301150.094.4158251
CGCGGTG351800.094.362895
GCGCGGT349250.094.045014
CGGGCAT2600.093.860511
GCGCTGT454.0563464E-1092.575774
CGGGAGG1116150.092.522921
CGGGTTT603100.091.852881
CGGGCGT465550.091.068691
CGGTAAT101200.090.458271
Produced by FastQC (version 0.11.5)