FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11465223
Sequences flagged as poor quality0
Sequence length125
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CNGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC403900.35228272489771895No Hit
CNGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT268290.23400329849667992No Hit
TNGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA266860.23275604844319206No Hit
TNGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG243490.21237266819842926No Hit
CNGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT237470.20712200713409587No Hit
TNGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA219280.19125663757259673No Hit
CNGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA195810.17078603704437323No Hit
CNGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA169030.14742844513360098No Hit
TNGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT151540.13217361755632664No Hit
CNGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT142350.12415807350628942No Hit
TNGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA135220.11793926729554235No Hit
CNGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT132090.11520927242322282No Hit
GNTCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT128930.11245311146586509TruSeq Adapter, Index 18 (97% over 40bp)
TNGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA118080.10298971071038042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAATT7200.096.631043
GGCGCGG243550.095.87873
CGCGGTG252900.092.033425
GCGCGGT250400.091.48134
CGGTGGT452800.090.139147
GCGGTGG373050.087.3578956
CTAATTT8850.078.615084
GGTGCGG109750.076.551633
ACGGTGG116500.073.170136
GTTAATT1425650.071.3368153
CGTAGTG138250.069.651535
GGTACGG122600.069.338773
GGAGGCG216700.069.203923
GTGCGGT117150.069.120944
GGCGGAG217950.067.84136
GCGGAGT120050.067.256427
GCACGGT702.055458E-1066.81814
GGCGTAG145600.066.537023
GGCGTGG196050.065.966053
GTACGGT127500.065.11494
Produced by FastQC (version 0.11.5)