FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18110107
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC710230.39217327650245243No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG526620.29078790092184437No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA519360.28677908970940924No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA470360.2597223749147368No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT337010.18608945822352127No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT200050.11046317948314717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC335550.0114.597081
CGGGTGC108100.0114.129211
CGGTTAA1210950.0113.264741
CGGGTAC135950.0107.52391
CGGCTAA7850.0106.950351
GGGCGCG388700.0102.535742
GGCGCGG403150.095.947663
CGGGTGT330700.095.7157751
CGGAATA161350.095.3947141
CGGGCGT469450.095.228661
CGGGTAT384200.095.0336761
CGCGGTG410500.094.097965
GCGCGGT404400.093.826644
CGGGCAT2800.093.568011
CGGGTTT896600.091.7720951
CGGTAAT132600.091.605041
CGGATAA99800.090.508171
CGGATGC13350.090.095451
CGGAATT220100.089.490761
CGGTGGT743850.088.6904147
Produced by FastQC (version 0.11.5)