FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18117009
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT839010.4631062445241375TruSeq Adapter, Index 18 (97% over 40bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC776470.42858619764443456No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA316250.1745597189911425No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT273910.15118941542723746No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT253950.14017214430925104No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG238830.13182639584712907No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA204900.11309813888153392No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT193630.10687746525930412No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA185380.10232373345953519No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC380300.0114.9427641
CGGGTGC116700.0113.483951
CGGTTAA990650.0112.836761
CGGGTAC150300.0109.5389251
GGGCGCG436050.0104.640192
CGGCTAA6200.0103.6829451
GGCGCGG454150.097.051993
CGCGGTG456150.096.332555
GCGCGGT451600.095.858294
CGGGTGT362650.094.719341
CGGAATA191150.094.443791
CGGGTAT429800.094.2126851
CGGATAC18850.093.466421
CGGTAAT134300.092.584381
CGGGTTT899300.091.211881
CGGGCGT578400.091.186331
CGGTGGT814200.089.001317
CGGAGTG108450.088.802311
GCGGTGG664350.088.441976
CGGAATT223150.088.4223251
Produced by FastQC (version 0.11.5)