FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005416168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25249714
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC863130.34183753526871635No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA450400.1783782580666062No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT437090.17310691123075692TruSeq Adapter, Index 14 (97% over 44bp)
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG434070.1719108580794222No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT410480.16256817799995674No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA307010.12158949602359853No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA289640.11471021018297474No Hit
TGGAGGAGGAGATTTTATTTTATATTAATATTTATTTTGATTTTTTGGTT257650.10204075974880349No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)