FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20370420
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC772160.37905944010972775No Hit
TGGAGTAGTGAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA403340.1980027903204745No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG327770.16090488070447248No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT319650.15691870859805543No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA261650.12844605069507647No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT254900.1251324224046436TruSeq Adapter, Index 18 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT245240.12039025214011297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC374200.0113.550751
CGGGTGC124650.0113.403731
CGGTTAA1155200.0113.232811
CGGGTAC144400.0109.026791
CGGCTAA8150.0103.74561
GGGCGCG440550.0101.8502962
CGGGTAT442100.095.2490541
GGCGCGG460650.094.667353
CGGGTGT390300.094.358291
CGGAATA196600.094.16211
CGCGGTG461500.093.9387365
GCGCGGT455300.093.637074
CGGGTTT974600.093.036931
CGGATGC13600.091.06751
CGGTAAT138150.089.391621
CGGAATT247850.088.889761
CGGGCGT601700.088.588841
CGGTGGT821500.087.942347
CGGGTAA103000.087.697521
CGGATAC18950.087.666551
Produced by FastQC (version 0.11.5)