FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22362829
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT669530.2993941419486774No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC648940.29018689898312955No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA506490.22648744485771455No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA452680.20242519405751394No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT355660.15904070097750156No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG262300.11729285234886874No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA250350.11194916349805295No Hit
AAGTAGAAGATGGTATATGAGATCGGAAGAGCACACGTCTGAACTCCAGT245120.10961046118091768Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT228250.10206669290365722TruSeq Adapter, Index 16 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA5350.0113.484921
CGGGTGC135500.0112.590321
CGGGCGC370300.0110.512341
CGGTTAA888350.0109.3991851
CGGGTAC138650.0105.438751
CGGGCAT4650.099.8466341
GGGCGCG451000.097.364892
CGGGTGT453050.094.373811
CGGGTAT493350.094.157281
GGGAGGC631250.092.634222
CGGATGC14050.092.3574451
CGGGTTT943450.091.300141
GGCGCGG464300.091.089183
CGCGGTG461950.090.453525
GCGCGGT462200.089.738914
CGGGCTA1000.089.285931
CGGTAAT138300.088.532731
CGGGAGG1464350.088.362191
CGGAATA246800.088.2729641
CGGAATG1267450.087.2300951
Produced by FastQC (version 0.11.5)