FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19717772
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC585370.29687431216873794No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT283190.14362170330400412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC312400.0114.207891
CGGTTAA1097500.0113.081371
CGGGTGC102400.0112.923581
CGGGTAC123450.0108.138381
CGGCTAA6550.0105.451321
GGGCGCG369050.0102.0567552
GGCGCGG382250.094.942123
CGGGTAT399050.094.5416641
CGCGGTG382200.094.311915
CGGGTGT340450.093.9720841
CGGATAC17600.093.3752441
GCGCGGT381700.092.9587864
CGGAATA165350.092.943521
CGGGCGT496150.092.9007951
CGGGTTT1026650.092.593791
CGGAATT222650.090.151181
CGGTAAT127750.089.024151
CGGATAA87400.088.021031
CGGAGTG108700.087.20651
CGGATAT144800.087.012661
Produced by FastQC (version 0.11.5)