FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14017297
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC582360.41545813005174964No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT469460.3349147842126767No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT465000.3317330010200968No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA396090.28257231048182824No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA391880.27956887836506567No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT374310.2670343647566289No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA350840.25029076575890485No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA289760.2067160309152328No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT283270.2020860369870168No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT258880.1846861060302853No Hit
TGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT237540.1694620581985243No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA230570.1644896302047392No Hit
CGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA217780.15536518916592837No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA204740.14606239705130025No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA199640.14242403510462823No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT185730.1325005812461561No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA185550.13237216847156766No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG174590.12455325730773914No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT169470.12090062727500173No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAATGGAA160540.11452992684680935No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAA150720.1075242965887075No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT142700.10180279407648993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC284950.0115.328981
CGGGTGC91850.0114.4460751
CGGCTAA8900.0113.0281
CGGTTAA705050.0112.048431
CGGGTAC107950.0110.169711
GGGCGCG327750.0105.055122
GGCGCGG341900.097.615233
CGGGTAT277250.097.5135351
CGGGTGT242350.096.573911
CGGGCGT352300.096.187331
CGCGGTG348850.095.977445
GCGCGGT342650.095.665354
CGGAATA201800.094.8012541
CGGTAAT91400.093.258021
CGGGAGG820400.093.0727841
CGGAAAT73900.092.2253651
CGGATGC8200.092.1890261
GGGAGGC341700.091.83362
CGGATAC13650.091.138731
CGGGTTT493050.090.1938861
Produced by FastQC (version 0.11.5)