FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20465760
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC682700.33358155279843016No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT263110.12856106980634974No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA253530.12388008068109858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC374050.0114.163651
CGGGTGC126050.0112.484931
CGGTTAA1066550.0112.181111
CGGGTAC158900.0110.591841
GGGCGCG438050.0102.4686052
CGGCTAA7350.098.843991
CGGGTAT449050.095.732661
CGCGGTG455450.095.260385
GGCGCGG455450.095.183633
CGGGTGT380100.094.2983861
CGGGCGT549050.094.283471
GCGCGGT452200.094.0785144
CGGATGC15700.094.0653841
CGGAATA206850.093.275431
CGGTAAT145550.090.827751
CGGGTTT916800.090.084141
CGGAATT241200.089.8179551
CGGTGGT846500.088.604427
GCGGTGG674600.088.069796
CGGGAGG1022350.087.720921
Produced by FastQC (version 0.11.5)