FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005416223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30069493
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC1001370.3330185846498975No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT501680.16684019248345824TruSeq Adapter, Index 13 (97% over 40bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT397880.13232015584699083No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA333320.11084989028581227No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT309990.10309119611694151No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)