FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20383885
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC656660.32214663691440565No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT291230.14287266632440282No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA285310.13996841132100185No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG257430.1262909401225527No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC241440.11844650811167744TruSeq Adapter, Index 4 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT225500.11062660528157414No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC361900.0114.228441
CGGGTGC117250.0112.872331
CGGTTAA1099500.0111.869271
CGGGTAC148050.0109.125681
GGGCGCG436200.0101.078422
CGGCTAA7350.0100.392181
CGGGCGT507350.095.403011
GGCGCGG447850.095.089973
CGGGTAT429150.094.886181
CGGGTGT366150.094.505311
CGCGGTG452050.093.86455
GCGCGGT453200.092.13064
CGGAATA168700.091.535141
CGGGTTT993050.090.7475741
CGGATGC14300.089.884181
CGGTAAT141150.088.321961
CGGTGGT820550.087.8003167
CGGATAC20400.087.2180941
CGGAATT234550.087.147881
CGGATAA96100.086.8761
Produced by FastQC (version 0.11.5)