FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17896260
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC472620.2640886978620114No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT212200.1185722603493691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA880300.0111.551991
CGGGCGC277700.0111.360981
CGGGTGC87800.0110.323441
CGGGTAC111950.0107.9291151
GGGCGCG333500.0101.1353762
CGCGGTG341200.094.841045
GGCGCGG345350.094.529723
GCGCGGT340750.093.552534
CGGGTAT363850.092.9720461
CGGGTTT905050.091.199411
CGGGCGT475400.091.118151
CGGGTGT321950.090.296991
CGGAATT188150.088.739671
CGGTAAT114050.088.641991
CGGAAAT86850.087.714211
CGGTGGT623200.086.580657
CGGAATA125450.086.4788061
GCGGTGG511900.086.385626
CGGATAA73450.085.213111
CGGAGTG100250.084.314211
Produced by FastQC (version 0.11.5)