FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15345699
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC404610.2636634538446245No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA281920.18371271324949093No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT237550.15479907432043338TruSeq Adapter, Index 16 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC84150.0111.914991
CGGGCGC256650.0110.8726651
CGGTTAA596000.0110.689891
CGGGTAC102750.0104.526021
GGGCGCG307450.098.105752
CGGGTAT318750.096.317391
CGGGTGT256400.094.299971
CGCGGTG307450.093.5142755
GCGCGGT307350.092.364014
GGCGCGG317550.091.682723
CGGCTAA4850.090.8867341
CGGGCGT367300.090.884061
CGGTAAT77750.089.2557141
GGGAGGC375850.089.024322
CGGGTTT542450.088.936941
CGGGAGG849050.088.5043261
CGGTGGT548350.088.111437
CGGGTAA71200.086.925281
GCGGTGG452850.086.515616
CGGATAC13150.085.614351
Produced by FastQC (version 0.11.5)