FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005416261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12257710
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC387020.3157359735219711No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC376500.30715362004811664TruSeq Adapter, Index 7 (100% over 50bp)
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT182800.1491306288042383No Hit
TGGGTGTGGTGGTTTATGTTTGTAATTTTAGTATTTTGGGAGGTTGAGGT150110.12246169961599679No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA146110.11919844734456925No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT144660.11801551839617677No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA138450.11294931924478553No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT131840.10755679486625153No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)