FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19108170
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC408970.2140288682799033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA902900.0112.287851
CGGGCGC279300.0111.2674561
CGGGTGC94000.0110.645981
CGGGTAC112800.0108.373881
GGGCGCG340150.0102.7741552
GGCGCGG356750.094.613433
CGGGTAT337100.094.098821
GCGCGGT351300.093.930674
CGCGGTG358550.093.8071065
CGGGCGT435150.091.8665541
CGGGTTT832900.091.132681
CGGGTGT313900.090.743141
CGGAATA159200.089.479381
CGGTAAT117050.088.6534041
CGGAATT194700.088.256511
CGGATAC17000.088.0020751
CGGTGGT660250.087.400277
CGGATAA74100.086.468581
CGGATGC13800.086.381161
GCGGTGG539900.086.1508566
Produced by FastQC (version 0.11.5)