FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17230972
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC430440.24980598889023786No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT199360.11569863847495081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA863500.0111.791231
CGGGCGC268800.0111.1485751
CGGGTGC94550.0109.99411
CGGGTAC107850.0106.542371
GGGCGCG322300.0100.564932
CGGGTAT348350.093.9615551
CGCGGTG329750.093.7329565
GGCGCGG333450.093.657833
GCGCGGT330050.092.6024254
CGGGTGT308700.092.477141
CGGAATA151100.091.902421
CGGGCGT447150.090.753961
CGGGTTT833050.090.494181
CGGAAAT88900.089.09611
CGGAATT195700.088.5907441
CGGTAAT110450.087.8464361
CGGATAA73250.087.139951
GCGGTGG500150.085.92946
CGGTGGT616350.085.612677
CGGAGTG103050.085.421651
Produced by FastQC (version 0.11.5)