FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19307105
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC722720.3743285179212523No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC624660.32353892517806265TruSeq Adapter, Index 12 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT461260.23890686874080813No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA305110.1580299066069201No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT245490.1271500828322009No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA238410.12348303901594776No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA238230.12338980908841589No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT201740.10449003100154063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC380700.0117.076731
CGGGTGC109800.0115.29471
CGGGTAC136900.0114.096911
CGGTTAA820350.0113.615651
CGGCTAA4650.0110.6683961
GGGCGCG452450.0105.773722
GGCGCGG470700.098.646013
CGCGGTG468050.097.949025
CGGGTAT447950.097.9053651
GCGCGGT464950.097.602674
CGGGTGT371800.096.675251
CGGGCGT588600.094.948061
CGGATGC11050.094.23751
CGGGCAT2250.094.1766361
CGGGTTT799350.094.0529251
CGGAATA218200.093.504561
CGGATAC15350.093.475461
GGGAGGC538850.093.415622
CGGTAAT125200.093.182641
CGGGAGG1235500.092.579711
Produced by FastQC (version 0.11.5)