FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8306663
Sequences flagged as poor quality0
Sequence length125
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC964891.16158558496956TruSeq Adapter, Index 12 (100% over 50bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC331790.3994263400357039No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT266870.32127221243958015No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA193530.23298164377199365No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA152540.18363571508799623No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT146940.17689413907847232No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA134900.1623997506579959No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT104810.12617581813539322No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT87770.10566216542069902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC155700.0113.8146061
CGGGTGC45350.0112.3400041
CGGTTAA392700.0110.053121
CGGGTAC62400.0107.5225141
GGGCGCG183900.0100.344812
CGGCTAA3100.096.106761
CGCGGTG187800.094.900195
CGGGTAT184850.094.222571
GCGCGGT185700.094.051354
GGCGCGG190650.093.543953
CGGGTGT154550.092.0684741
CGGGCGT228200.091.363751
CGGGAGG539850.089.106111
CGGGTTT377500.088.897861
GGGAGGC222300.088.663242
CGGTGGT329100.087.377517
CGGAATA98450.087.276071
CGGTAAT50050.087.0279851
GCGGTGG267250.086.7205666
CGGGGAC15000.085.8041151
Produced by FastQC (version 0.11.5)