FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005416302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11885868
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC502940.42314116226093035No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT352090.296225736311391No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA256680.21595393790339923No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA243820.20513436629112825No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT187170.1574727230691103No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT176980.14889951663605888No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG165980.13964482863178357No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT145880.1227339896421532TruSeq Adapter, Index 18 (97% over 40bp)
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA137730.11587710716625828No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA129030.10855749029014962No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT125920.10594093759075905No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)