FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18523703
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA589320.3181437318445453No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC492120.26567042237721045No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT420140.22681210123051532No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA378770.204478553775128No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT321370.17349122904853312No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT268720.14506818642039337No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG251390.1357126056275033No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA246980.13333187214241127No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA242130.13071360515767286No Hit
CGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA197260.10649058668237123No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA188980.10202063809811676No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT186680.10077898571360165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1306800.0113.7395861
CGGGTGC93150.0111.789471
CGGGCGC264550.0110.213311
CGGGTAC103750.0105.357831
CGGAATA366650.0103.1845251
GGGCGCG310050.0100.711372
CGGATAA99650.093.271611
GGCGCGG324400.093.219163
CGCGGTG322750.093.05125
CGGTAAT115950.092.88671
CGGATGA88950.092.650881
CGGGTGT283800.092.48481
GCGCGGT322000.091.6803054
CGGGCGT392900.091.641211
CGGGTAT303200.091.2969361
CGGAATT237850.090.4630051
CGGGTTT787250.090.1270141
CGGATAC14800.086.843591
CGGAGTG100750.085.933731
GCGGTGG491950.085.517686
Produced by FastQC (version 0.11.5)