FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22418689
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC678950.30285000162141507No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA363170.1619943075172683No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT324340.1446739369996167No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT305950.13647095956413866No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA272150.12139425280398868No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA249580.11132675956207787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC347000.0113.036181
CGGGTGC112650.0113.0176851
CGGTTAA1251200.0112.1337051
CGGGTAC141400.0107.667531
GGGCGCG407150.0101.991482
CGGAATA284150.097.7761841
GGCGCGG424400.094.804053
CGCGGTG424700.093.770525
GCGCGGT421900.093.250654
CGGGTTT1199950.092.5348741
CGGGTGT416600.092.2237241
CGGGTAT458800.092.18261
CGGGCGT603900.089.6873251
CGGAATT262800.089.465471
CGGATAA97950.087.644721
CGGGTAA102850.086.997631
CGGATAC19650.086.589921
CGGTAAT138900.086.476461
CGGTGGT782300.085.980227
GCGGTGG636900.085.647296
Produced by FastQC (version 0.11.5)