FastQCFastQC Report
Wed 11 May 2022
EGAF00005416323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23805110
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC648460.2724036981975719No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT345430.14510750002835526No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA312960.13146757145839696No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG287980.12097402616497047No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC267670.11244224454329343TruSeq Adapter, Index 12 (100% over 50bp)
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA261750.10995538352899861No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)