FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18980862
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC614880.3239473528652176No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT291600.1536284284665259No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA201260.10603311904380318No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG194080.1022503614430156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC328600.0113.908811
CGGGTGC108150.0113.478161
CGGTTAA1182300.0112.790511
CGGGTAC131600.0108.529111
GGGCGCG378500.0104.150082
GGCGCGG391300.097.675063
CGCGGTG393150.096.805635
GCGCGGT387000.096.518674
CGGGCGT492450.094.1202551
CGGGTAT401950.093.802591
CGGGTGT338200.093.656561
CGGGTTT955450.092.304071
CGGAATA167800.091.068941
CGGAATT233450.090.317931
CGGTAAT135250.090.213011
CGGAAAT109300.089.925281
CGGATAC18450.089.593711
GCGGTGG583200.089.198666
CGGATAA97950.088.932861
CGGTGGT737600.088.2684567
Produced by FastQC (version 0.11.5)