FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21875550
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC642040.2934966206563949No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT311860.14256098703804018No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA250290.11441540898400268No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC122700.0113.107131
CGGGCGC337250.0112.716231
CGGTTAA1215650.0112.2262041
CGGGTAC146900.0108.0744861
GGGCGCG398700.0102.312432
CGCGGTG411050.095.3210145
CGGAATA214700.094.800061
GCGCGGT406300.094.725914
GGCGCGG417050.094.56323
CGGGTAT475000.094.1615141
CGGGTGT416050.092.3111
CGGGTTT1164400.092.1740651
CGGGCGT586250.091.9431
CGGAATT248000.089.671341
CGGTAAT141150.087.7489851
CGGATAA89200.087.525291
CGGATAC19300.087.4973141
GCGGTGG627900.087.211386
CGGAAAT116250.086.8005751
CGGTGGT783950.086.782427
Produced by FastQC (version 0.11.5)