FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19864391
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA509020.25624747317952007No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT425620.21426279818998728No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA383010.19281235452926798No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC329550.16589987581295595No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT282470.14219917439200627No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT279350.14062852468016765No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG206660.1040354068745425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1333900.0112.866011
CGGGTGC106600.0110.331291
CGGAATA366400.0102.065881
CGGGCGC221350.0101.213651
CGGGTAC102950.0101.118921
CGGATGA107650.093.3866041
CGGATAA111000.093.24931
CGGGTGT326650.092.638581
CGGTAAT134350.091.30811
CGGAAAT118500.089.90911
CGGAATT265100.089.6068041
CGGGTTT863600.088.4679641
GGGCGCG275800.088.331672
CGGGTAT321550.088.3140641
CGGGAGG758850.086.985041
GGGAGGC286200.086.702422
CGGGCGT350450.086.6529541
CGGTATT366650.085.648961
CGGGTAA91300.085.337091
CGGATGC12350.083.377351
Produced by FastQC (version 0.11.5)