FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20254348
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC478070.23603327048592232No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC320520.15824750320276912TruSeq Adapter, Index 12 (100% over 50bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT255190.12599270043153205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1050500.0111.368161
CGGGCGC299550.0109.215321
CGGGTGC101200.0108.640691
CGGGTAC122750.0103.776361
GGGCGCG369850.095.949372
CGGGTAT379550.091.386521
CGGGCGT480250.090.9156951
CGCGGTG371050.090.875585
GGCGCGG381550.089.887823
CGGGTGT339550.089.231911
GCGCGGT375500.089.0861364
CGGGTTT1056550.089.033951
CGGTAAT130950.086.86831
CGGAATT219550.086.489491
CGGAAAT107600.085.195091
CGGAATA141250.084.705911
CGGTGGT680900.084.506717
CGGATAA84850.083.764221
GCGGTGG564950.082.756026
CGGAGTG112200.082.4451
Produced by FastQC (version 0.11.5)