FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12897236
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC227640.17650293442719045No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA172190.13350922631794906No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT145180.11256675461315897No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG134410.10421612816885727No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT132770.1029445378839311No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA129700.10056418289934371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA730000.0111.903211
CGGGTGC63500.0108.539531
CGGGCGC173200.0104.8105551
CGGGTAC74550.0102.031971
CGGGCGT255950.093.318361
GGGCGCG210300.093.073162
CGGGTAT221350.092.040821
CGGAATA99350.089.6224441
CGGGTGT200000.089.307731
CGCGGTG210950.088.887535
GCGCGGT208600.088.155784
GGCGCGG215350.087.602023
CGGGTTT582000.087.488331
CGGATAA53700.087.3383561
CGGAATT129700.086.1804351
CGGTAAT74850.084.765371
CGGAAAT64100.084.031791
CGGTGGT402700.083.978447
CGGATAC11500.083.326111
CGGGAGG441550.081.186921
Produced by FastQC (version 0.11.5)