FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005416361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12057433
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC480560.39855912945981126No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT350360.2905759459745702No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA231890.1923212013701424No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT187480.15548914930731939No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC167280.13873599795246633TruSeq Adapter, Index 5 (100% over 50bp)
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT146400.12141887912626179No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT145610.12076368162277991No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA143440.1189639618980259No Hit
CGGTGGATTTTTCGGTTTAAGTTTTGGTAATACGGTGAAATTTCGTTTTA126950.10528775071775229No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)