FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18229820
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT485240.26617926013531673No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC438260.24040829805231212No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA385140.21126922811086452No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA369420.20264599431042107No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA257700.14136179073627717No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT257700.14136179073627717No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT223830.12278234233799347No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA746750.0109.636841
CGGGTGC106600.0109.1592941
CGGGTAC102600.0103.7268751
CGGGCGC252650.0103.1401141
GGGAGGC641150.0100.270882
CGGCTAA6500.098.896641
CGGGAGG1480600.097.169071
CGGAATA295400.094.540661
CGGGTTT682800.093.169571
CGGGTGT344050.093.0747451
GGGCGCG303000.092.112872
GCGGAGT418700.091.981627
CGGGTAA106150.091.6787951
CGGTAAT130350.091.4620741
CGGGTAT354500.090.935521
CGGGAAG195350.087.598281
CGGGATA86850.087.516961
GGAGGCG741800.086.286343
CGGATGA61550.086.25971
CGGAGTG115900.086.226031
Produced by FastQC (version 0.11.5)