FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11348483
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC497570.4384462663423825No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT402680.3548315664745676No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA322790.28443449225768763No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA239680.21120003440107368No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT236970.20881205003347145No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT162530.1432173798030979No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT124780.10995302191491145No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA123340.1086841298524217No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA122740.10815542482638428No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT121600.10715088527691323No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT120430.10611991047614029No Hit
CGGTGGATTTTTCGGTTTAAGTTTTGGTAATACGGTGAAATTTCGTTTTA118210.10416370187980191No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC76350.0113.979961
CGGGCGC243950.0112.756211
CGGGTAC92950.0110.157431
CGGTTAA510600.0109.913141
GGGCGCG275600.0103.152662
CGGCTAA4550.0100.801421
CGGGTAT270100.098.178851
CGGGTGT232850.096.0296551
GGCGCGG287300.095.916183
CGGAATA177050.095.646321
CGGGAGG904400.095.2544251
CGCGGTG288050.094.426975
CGGGCGT331700.094.3478241
GCGCGGT286850.093.950744
GGGAGGC385550.093.524332
CGGTAAT78650.093.000861
CGGGTAA66750.092.626111
CGGATGC7250.091.1952741
CGGGTTT481300.091.011251
CGGATAC9700.090.2678151
Produced by FastQC (version 0.11.5)