FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22031558
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT1185300.5380009893081551No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA914610.4151363239948805No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT720310.32694464912558613No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA647220.2937695100818562No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT423730.1923286587358007No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA383850.17422735151095534No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA374590.1700242897029797No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA350930.15928514905754737No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT350250.15897650089022303No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC342200.15532265126233924No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA338640.15370678732752355No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA309370.14042129930166536No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT285850.12974570386715276No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGAT264080.1198644235691366No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA253820.1152074673974487No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT243250.11040980397300998No Hit
CGGAATAGAATGGAATGTAATGGAATGGAACGGAATGGAATGGAATGGAA230610.10467257921568689No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA230510.10462718977931565No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA229740.1042776911192572No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG225270.10224878331346335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC85450.0109.608611
CGGTTAA696650.0106.021781
CGGCTAA6450.0104.904691
CGGGCGC207100.099.276761
GGGAGGC666250.098.928182
CGGGTAC87600.097.703681
CGGGAGG1480500.097.018981
GCGGAGT464850.093.880317
CGGAATA439800.091.905531
CGGGTAA104700.090.759991
CGGGTGT328250.090.391621
CGGTAAT124650.089.75711
CGGGAAG209100.088.988371
CGGGTTT717900.088.935881
CGGAGTG170700.088.352921
GGGCGCG255950.087.845442
CGGATGC9550.087.632561
CAGAAGA25850.087.368724
CGGATTG168250.087.311191
CGGGTAT337250.087.099421
Produced by FastQC (version 0.11.5)