FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005416401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14105041
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC439423131.153620893409666TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAGATCATCTCGTATGC876150.6211609026872024TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAGATCATCTCGTATGC822050.5828058209827253TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCAGATCATCTCGTATGC775570.5498530631708196TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAGATCATCTCGTATGC752360.5333979532565697TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAGATCATCTCGTATGC744610.5279034637332851TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCAGATCATCTCGTATGC718040.5090662267482952TruSeq Adapter, Index 7 (98% over 50bp)
GATCTGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC673310.4773541601190667TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC602350.42704590507748263TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCAGATCATCTCGTATGC576000.40836464069831485TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATTTCGTATGC420420.2980636497263638TruSeq Adapter, Index 7 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC333810.2366600706796953TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC296560.21025107264842405TruSeq Adapter, Index 7 (98% over 50bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC276550.19606465518249824No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT235010.16661419133769267No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA213710.15151320722853623No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA204800.1451963166927342No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT156270.11079017778112095No Hit
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC147300.10443074926191281TruSeq Adapter, Index 7 (98% over 50bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA143510.10174376664342911No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)