FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005416405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30527187
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT858320.2811657687293625No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA735570.24095570941403804No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA696010.22799676891290377No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC681380.22320431948086145No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT580400.1901256083634565No Hit
AAGTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAA450600.14760613219947188TruSeq Adapter, Index 13 (97% over 40bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA438320.14358348838364965No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA342640.11224093461346439No Hit
AAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAA320060.1048442491605925TruSeq Adapter, Index 13 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT307950.10087729341062444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)