FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16501775
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT1043990.6326531539788902No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA794800.4816451563543922No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC745040.45149082447191285No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT628320.3807590395578658No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA583490.3535922650745147No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT439630.266413764579871No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA330940.20054812285345058No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA307050.18607089237369917No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA303640.18400444800635082No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA287370.17414490259381188No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT257390.15597716003278436No Hit
CGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA220220.13345231043327158No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGAT216200.13101620886237997No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAG211890.1284043686209514No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT206610.1252047128263475No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT193800.1174419115519391No Hit
CGGAATAGAATGGAATGTAATGGAATGGAACGGAATGGAATGGAATGGAA184070.11154557615771638No Hit
CGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGAT182640.11067900271334447No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT182350.11050326404280751No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT175560.10638855517058013No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA172140.10431605084907532No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA167150.10129213372500837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC105500.0115.768511
CGGGCGC343050.0115.4145051
CGGTTAA792050.0112.3676451
CGGCTAA8100.0110.2228851
CGGGTAC130450.0109.641571
GGGCGCG394400.0104.4143452
CGGGTAT348150.097.858551
CGGAATA383600.097.597261
GGCGCGG410800.097.168013
CGGGTGT307950.096.774941
GCGCGGT408200.096.0381244
CGCGGTG415300.095.241385
GGGAGGC497500.094.829962
CGGGAGG1162650.094.452381
CGGATAC14250.093.979521
CGGGCGT452450.093.888121
CGGTAAT112350.091.651281
CGGGTAA88650.091.378691
CGGGTTT598650.091.1124341
CGGTGGT723500.090.346247
Produced by FastQC (version 0.11.5)