FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21230811
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT1423410.6704454201019452No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA1071980.5049171225724726No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA767760.3616253754979026No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT758730.35737212299614934No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT557380.2625335414648079No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC509820.2401321362617754No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA503030.236933954148054No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA416600.19622425163127308No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA408840.19256918635844858No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA406340.1913916524432345No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA361610.17032321563222433No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGAT331180.15599027281623862No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAG292350.13770081604513365No Hit
CGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTCGAT289900.13654683280822386No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA285160.13431422850497798No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT273690.12891170290197582No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG242340.11414542760519134No Hit
CGGAATAGAATGGAATGTAATGGAATGGAACGGAATGGAATGGAATGGAA223500.10527153202013809No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT222380.10474399682612219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA833150.0110.663941
CGGGTGC107550.0110.4067461
CGGCTAA6900.0107.934331
CGGGCGC282300.0104.32261
CGGGTAC105050.0103.5061
GGGAGGC592200.098.327882
CGGGAGG1377250.097.317561
CGGAATA507600.094.33461
CGGGTGT340550.093.441631
CGGGTAA111250.092.00721
CGGGCAT3500.091.923051
CGGTAAT137000.091.891981
GCGGAGT386000.091.721017
GGGCGCG340600.091.607572
CGGATGA79600.091.390551
CGGGTTT630400.090.8441
CGGGTAT353500.090.7938161
CGGATGC10900.090.189541
CGGGAAG199800.089.339811
CGGATTG161300.088.5385361
Produced by FastQC (version 0.11.5)