FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15450291
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT894030.5786492953433693No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC661860.42838028099276576No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA598090.3871059774861198No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA515790.33383837236463704No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT484440.31354749240645374No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT332610.2152774986568214No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA252660.163530900485952No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA224790.1454924052886771No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA224500.1453047065586014No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT223040.1443597405382203No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA204850.13258649950347215No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT200010.12945387242220874No Hit
CGGTGGATTTTTCGGTTTAAGTTTTGGTAATACGGTGAAATTTCGTTTTA188400.12193945084917818No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGAT174570.11298816313556811No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAG173060.11201083526517397No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT164650.10656757209297871No Hit
CGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA160780.10406276490196852No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT159180.10302718570155087No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT159020.1029236277815091No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC295450.0114.8069461
CGGGTGC93850.0114.090741
CGGTTAA787300.0112.543511
CGGCTAA8350.0111.196641
CGGGTAC116200.0109.510341
GGGCGCG340850.0103.819042
CGGAATA314250.097.9383541
CGGGTAT328550.096.284261
GGCGCGG355750.096.2520753
CGGGTGT280500.095.930161
GCGCGGT353550.095.3534854
CGGATAC11750.095.2299351
CGCGGTG360050.095.168655
CGGGAGG1149450.095.016611
GGGAGGC483100.093.6304862
CGGGCGT419750.092.649261
CGGTAAT104900.092.0866241
CGGAGTG121700.091.4543841
CGGGTTT585150.091.289491
CGGGTAA85150.090.658561
Produced by FastQC (version 0.11.5)