FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19992102
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT372410.1862785614038984No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC340100.17011717927409534No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA328630.16437991362789167No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT301510.15081455666842838No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT250480.125289476814394No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT211920.10600186013456714No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT207400.10374096730798993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC94600.0106.388661
CGGTTAA641000.0105.496871
CGGGTAC94700.0100.054341
CGGCTAA5100.098.028411
GGGAGGC629850.096.431532
CGGGCGC224450.096.389041
CGGGAGG1471500.093.549061
CGGGTAA110150.091.909971
CGGGTGT371150.091.821571
CGGGTTT776200.091.070121
CGGTCTA1050.090.692951
CGGTAAT129700.089.207041
CGGGTAT382550.089.069731
CGGAGTG141050.088.948851
GCGGAGT421600.088.076397
CGGGAAG202350.086.856651
CGGAATA199000.086.1355061
GGGCGCG281000.085.870232
CGGGGAC27050.085.37041
CGGATGA58700.083.2430341
Produced by FastQC (version 0.11.5)