FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22579406
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT430460.19064274764358283No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC417140.18474356677053416No Hit
TGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT353310.15647444401327476No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA348870.1545080503889252No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT310040.13731096380480515No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT257010.11382496067434192No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT247680.1096928767745263No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA245140.10856795789933535No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA227100.100578376596798No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC118100.0110.17561
CGGTTAA834700.0107.563651
CGGGCGC266250.0103.5139161
CGGGTAC120900.0101.119231
GGGAGGC627800.095.149242
CGGCTAA5100.093.4549941
CGGGTGT437550.093.0529251
CGGGTTT1020400.090.773451
CGGGAGG1495950.090.739341
CGGGTCA1650.090.269031
CGGATGC12250.089.974271
CGGGTAT449750.088.992121
CGGTAAT145150.088.863531
GGGCGCG335300.088.685872
CGGGTAA124700.088.00471
CGGAATA230650.087.2805561
CGGAGTG145450.086.714131
CGGAGTA166400.085.964971
GCGGAGT433900.084.462267
CGGGAAG216950.083.565121
Produced by FastQC (version 0.11.5)