FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22443505
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT947930.4223627281032976No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA715740.31890740773332865No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT565030.25175657723693334No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC529250.23581432579269593No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA516750.23024478574090812No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA511340.22783428880649437No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT380690.16962145618520816No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA348580.15531442170017562No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT326670.14555213189740196No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA278120.12392003833625809No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA268360.11957134146382217No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT265200.1181633617387302No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA247990.11049521899542875No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG246930.11002292199903713No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA226280.10082204183348369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC120850.0109.169531
CGGTTAA713850.0106.9016341
CGGGCGC306600.0104.904951
CGGGTAC122450.0104.484021
GGGAGGC768150.096.2467652
GGGCGCG364650.094.7896652
CGGGAGG1764350.094.477951
CGGGTGT440900.094.214171
CGGCTAA5050.093.177291
CGGGTAA127450.092.0663151
CGGGTAT462400.092.04911
CGGGGAC31750.091.173141
CGGGTTT809750.090.9457551
GCGGAGT518050.090.505877
CGGTAAT145750.089.824231
CGGAATA357300.089.519091
CGGAGTG161700.088.66261
CGGATGC11850.087.961791
CGGGAAG227800.086.886241
GGCGCGG390250.085.173173
Produced by FastQC (version 0.11.5)