FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22578711
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT1367680.6057387421274846No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA1114110.4934338368563201No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA905290.40094848638613606No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT845770.3745873712631337No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT508340.22514128463755081No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA504760.22355572025347237No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT486180.21532672967912117No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA457330.2025492066398299No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA391300.17330484455024914No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT378150.16748077425677665No Hit
CGGAATAGAATGGAATGTAATGGAATGGAACGGAATGGAATGGAATGGAA354810.1571436031047122No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGAT340040.1506020427826903No Hit
CGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA336020.1488216045636972No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA335080.14840528318910676No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC334910.148329991025617No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA305510.13530887569268235No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA299610.1326957947245084No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA270170.11965696358839971No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT268170.11877117342969667No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAG264980.11735833812656533No Hit
CGGAATTGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAATGGAA262780.11638396895199199No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT257770.11416506460444088No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA236940.10493956010154877No Hit
TGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT235580.10433722279363068No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATTTCGTTATTGTATTTTAGTT227680.1008383516667537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC90400.0107.285981
CGGTTAA689500.0107.181411
GGGAGGC673000.099.69262
CGGGTAC87200.099.4722441
CGGGCGC203200.099.094611
CGGGAGG1584600.094.5642851
CGGATTG197000.093.171381
CGGAATA598600.092.9538041
GCGGAGT475100.092.1881267
CGGGTGT361850.090.270671
CGGAGTG201450.090.107761
CGGGTTT782800.090.076421
CGGTAAT122500.089.920321
CGGGTAA111300.088.906081
CGGCTAA4550.087.72441
CGGGAAG227200.087.3944851
CGGAGTA151550.087.071261
GGGCGCG254600.086.953212
GGCGGAG800050.086.244256
CGGATGC9550.085.462231
Produced by FastQC (version 0.11.5)