FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005416424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32008777
Sequences flagged as poor quality0
Sequence length125
%GC28

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAAGATGGTATATGAGATCGGAAGAGCACACGTCTGAACTCCAGT698170.21811829924023649Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT585390.18288421328937374TruSeq Adapter, Index 16 (97% over 40bp)
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA351330.10976051974744302No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)