FastQCFastQC Report
Wed 11 May 2022
EGAF00005416426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22981044
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT890270.3873931924067505No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC622440.2708493138954No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA570000.24803050723021985No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA529570.23043774686650442No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT442060.19235853688805435No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT386880.16834744322320605No Hit
AAGTAGAAGATGGTATATGAGATCGGAAGAGCACACGTCTGAACTCCAGT364470.15859592801789163Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA306220.13324895074392618No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA266660.1160347632596674No Hit
CGGGAGGCGGAGTTTGTAGTGAGTCGAGATCGCGTTATTGTATTTTAGTT261810.11392432824200677No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA259310.11283647514011984No Hit
CGGTGGATTTTTCGGTTTAAGTTTTGGTAATACGGTGAAATTTCGTTTTA247600.10774097121088146No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA246380.10721009889716063No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)