FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005416427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17063519
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC629080.3686695575514054No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT580890.340428020738278TruSeq Adapter, Index 16 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT227180.1331378363396202No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA197800.1159198170084377No Hit
CGGTGGATTTTTCGGTTTAAGTTTTGGTAATACGGTGAAATTTCGTTTTA183200.10736355144563088No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT172600.10115146822879853No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA171230.10034858577530226No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)