FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15643202
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC595420.38062539881540874No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT308580.19726140466638478No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT242090.15475731886604802No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA224360.14342332215616727No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA182050.11637642983834129No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA176180.11262400114759114No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT169480.10834099054656457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC284800.0115.410091
CGGGTGC95300.0113.1131061
CGGTTAA923650.0112.196351
CGGGTAC119900.0106.2881551
CGGCTAA6950.0104.487041
GGGCGCG332750.0103.345512
CGGATGC10100.097.241121
GGCGCGG346050.096.028053
CGGAATA181450.095.526041
CGCGGTG351100.094.51135
CGGGTAT339600.094.473171
CGGGTGT294650.094.17881
CGGGCGT422900.092.810831
GCGCGGT351700.092.7431644
CGGGCAT3750.092.062781
CGGGTTT796050.091.059031
CGGTAAT107050.090.1885761
CGGATAC16150.087.718631
CGGGTAA82100.087.508751
CGGAATT201400.087.4821851
Produced by FastQC (version 0.11.5)