FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24661474
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA685390.27791931658261787No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT367620.14906651565109208No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG357290.1448777960311699No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC346350.140441727043566No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA328390.13315911287378848No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA312920.12688617071307257No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA307550.12470868529593973No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT281890.11430379222263844TruSeq Adapter, Index 19 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT273670.11097065812043513No Hit
CGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT266600.10810383839992696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC104250.0105.892951
CGGTTAA660700.0102.0230261
CGGGTAC103700.095.629671
CGGGCGC243000.094.663081
GGGAGGC576500.093.794682
CGGGTTT870950.089.325031
CGGGTGT418650.088.885711
CGGAATA253700.088.1624761
CGGGAGG1387500.086.649481
CGGTAAT137050.086.638051
CGGGTAT431550.086.0896151
GGGCGCG311950.085.133192
CGGGTAA118050.084.716971
GCGGAGT390400.084.2583167
CGGAGTG132650.083.9003751
CGGCTAA6050.082.353891
CGGTATT350050.080.341681
CGGAGTA135950.079.567731
CGGGAAG198000.079.3717351
CGGATGA64500.078.456561
Produced by FastQC (version 0.11.5)