FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19119628
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC950820.49730047049032544No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC535080.279859001440823TruSeq Adapter, Index 4 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT346290.18111754057139606No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA285480.149312528465512No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA255410.1335852350265392No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT233880.12232455568696211No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT231070.12085486182053333No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT210190.10993414725432941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC444650.0116.369421
CGGGTGC138050.0114.476321
CGGCTAA8150.0112.473621
CGGTTAA903500.0111.4572751
CGGGTAC162500.0111.134711
GGGCGCG502500.0106.514132
GGCGCGG523700.099.2934653
GCGCGGT519000.098.28994
CGGGTAT486500.097.867931
CGCGGTG530500.097.740375
CGGGTGT412700.097.037381
CGGGCGT634850.094.894231
CGGATGC15300.092.980961
CGGGAGG1441450.092.973651
CGGAATA206400.092.543851
GGGAGGC599800.092.528212
CGGGTTT832850.092.267121
GCGGTGG758500.091.535856
CGGTGGT933050.091.231137
CGGTAAT136350.090.452831
Produced by FastQC (version 0.11.5)