FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13800189
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC644290.4668704174993546No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC636580.46128353749358075TruSeq Adapter, Index 4 (100% over 50bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA574310.41616096707081335No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG383800.2781121331019452No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA340100.24644590012499104No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT256490.1858597733697705No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT205020.14856318272162794No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA204130.1479182640179783No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA197140.14285311599717945No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT155750.11286077313868673No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT153930.11154195062111107No Hit
TGGTTAGTGGGGGTAGGTTTTTTAGGGAGAGGAGGGTGGATGGAATTAAG143120.10370872456891714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC296100.0114.329291
CGGGTGC94950.0113.907621
CGGTTAA752800.0113.250491
CGGGTAC110550.0111.740771
GGGCGCG336850.0104.80712
CGGCTAA5200.0104.281961
GGCGCGG354150.096.751383
CGGGTAT324600.096.470671
CGCGGTG351900.096.169155
GCGCGGT349700.095.9742364
CGGGTGT289250.095.858541
CGGAATA164600.095.394191
CGGGTTT616250.092.519941
CGGTAAT100350.092.2793961
CGGATGC10750.092.017571
CGGATAC13050.091.781831
GGGAGGC332700.091.637772
CGGGCGT425300.091.283041
CGGAGTG89850.090.3959351
GCGGTGG509750.089.75076
Produced by FastQC (version 0.11.5)