FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14027807
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC644550.4594802309441526No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT477120.3401244399783943No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC453460.3232579404606864TruSeq Adapter, Index 4 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA363610.25920658874191815No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA335090.2388755419859997No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA328290.23402802733171338No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT242850.17312043144021014No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG215390.1535450266745187No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT205440.14645197214361447No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA205150.1462452399010052No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT202410.14429197664324864No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA158700.11313243759341714No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT156490.11155699533077408No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA142440.10154117461125606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC96600.0114.320881
CGGGCGC305250.0113.6276551
CGGTTAA772900.0112.803271
CGGGTAC109850.0110.563081
CGGCTAA5150.0105.2512051
GGGCGCG349100.0103.5653842
CGGAATA229000.097.541341
GGCGCGG361450.097.027813
CGGGTAT316450.096.053541
GCGCGGT360100.095.491324
CGGGTGT283450.095.447291
CGCGGTG363600.095.4387745
CGGTAAT100650.092.3216861
GGGAGGC361200.091.760162
CGGGAGG883900.091.6559451
CGGGCGT410000.091.6432951
CGGGTTT603200.090.7797241
CGGATAC12400.090.308591
CGGATGC9650.089.5021741
CGGAATT196850.089.446211
Produced by FastQC (version 0.11.5)