FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12103894
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1427941.1797360419712863TruSeq Adapter, Index 4 (100% over 50bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC453780.37490414241896036No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT194410.16061773178119373No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT184310.15227330972991007No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA145920.12055624413102098No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA142180.11746632943084266No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA124690.10301643421530295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC223050.0114.032241
CGGTTAA691700.0112.914181
CGGGTGC63250.0110.699431
CGGGTAC82100.0110.084771
GGGCGCG261650.0101.743912
CGCGGTG264700.096.4565355
GGCGCGG269750.095.951593
CGGGTAT276200.094.567651
GCGCGGT266950.094.462774
CGGCTAA3750.093.6740651
CGGAATA108200.092.9396441
CGGGTGT235450.092.3992541
CGGGTTT688000.092.224261
CGGATAC11500.091.637671
CGGGCGT352500.090.042631
CGGAAAT62700.088.6906741
GCGGTGG380350.087.486586
CGGTAAT74950.087.4609761
CGGTGGT463200.087.38877
CGGATAA50550.086.8050461
Produced by FastQC (version 0.11.5)